I want to put larger tires on my road bike. I can fit a larger tire in the rear (28) than I can in the front (25). Is there any advantage/ disadvantage to doing this?

More of your weight is on your rear wheel, so it needs more air pressure than the front to prevent pinch flats. But larger tires require less pressure to prevent pinch flats. So it can make sense to run a larger tire on the back for lower pressure and thus a nicer ride. I'm currently running a 25 in back and 23 in front.

Cant think of any disadvantages -- maybe the sensitive would want to readjust their saddle angle.

My fixie commuter is running 23 front, 28 rear. It's held up quite nicely, and it seems to be a bit more compliant over the cobbles on my commute to class (it's probably the frame though). I say that if it fits, give it a try, you're not hurting anything by doing it.

I've done that with no noticable difference. It works well in my opinion. I ran a 25 on the front and a 28 on the rear.

Wider tire in front = better handling
Wider tire in back = comfyer ride

Cool. 25 F / 28 R is what will fit my bike. Thanks!